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The Essence Of Real Time PCR Kit In Research

By Audra Olson


The testing package that includes the method used to analyze for messenger ribonucleic acids or RNAs are called real time PCR kit. The formal name of this method is reverse transcriptase PCR. Many experts in the medical field has contradicted the use of the term real time PCR. However, this was used often that it is sometimes accepted as such.

This is a series of kits that are utilized extensively by researchers in the laboratories and used mainly for fast production of copies of genes. It is also used to identify the types of RNAs existing in the sample, as well as the quantity of the nucleotides. The samples utilized for testing may be large or small. It can be a biopsy sample removed from the infected body part of the patient, or a single cell sample extracted from various samples inside the laboratory. The many applications of the tests are used mainly in the study of body functions. The results are employed in large and continuing research that involves assays of genome, analysis of the different strands of genes, cloning, and in diagnosing a disease.

To diagnose a disease, this method can be utilized to test even samples of minute sizes and quantity. Therefore, the process makes it easy for the technicians to perform the analysis even for the most difficult to collect samples. A bone marrow from persons inflicted with leukemia is a good example of the sample. Screening is important to determine the specific type of leukemia that has infected the marrow for the attending physicians to determine correctly the path for treatment.

Once a signature is determined, it is employed by the attending physician to plan for the possible treatment. Many medical conditions have signatures that are similar to one another. Almost all these are already on record and studied extensively. The data from the studies such as the different characteristics of the individual signatures are also used in the research for new drugs and therapies that can be utilized as cures.

Study of genetic signatures are also using PCR kits extensively. Several of the procedures used in the tests were utilized in identifying nucleotides present in the sample. There are also extensive tests that quantify the nucleotides of different types.

Nucleotides can also be inserted in a chain or deleted using a specific kit. This creates a new signature for the genes. This is usually done in the formulation of new tissues that are resistant to cancer cells and the developing of new treatment techniques.

Analysis of the composition of the genome is another application of the PCR kit. Genome is the term utilized to describe the sets of genes that composes the biological organism. The analysis result creates links between genes in a particular genome.

Another goal of the genome study is to determine the relationship between different body functions and how the others are affected by the action of one organ. Information gathered in the process is used in computations and formulations of new technology. These are the technologies used in advanced applications to processes inside the living organism.

Cloning is a process that duplicates existing cells and follows closely the DNA composition of the original sample. Here, DNA stretches are copied and reproduced. Real time PCR kit is used in analysis related to cloning studies and in the manufacture of new cell vaccines.




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